| 1. | Collection and preservation of samples : as soon as the three vital signs disappeared , the dogs were anatomized , and the heart , liver , kidney , spleen , lung , brain , muscle in the injection location and no injection location , the heart blood , urine , bile , cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space , spinal cord ( medulla oblongata , cervical cord , the upper beast spinal cord , breast spinal cord and waist spinal cord ) were taken out , some of which were preserved at - 20 for qualitative and quantitative analysis , and the others were fixed with 4 % formaldehyde for the pathology observation
3 、样品采集：当心电、血压和呼吸全部消失时，迅速解剖动物，采取心脏、肝脏、肾脏、脾脏、肺脏、大脑、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、胆汁、侧山西医科大学硕士学位论文脑室脑脊液、脊髓腔脑脊液和不同节段的脊髓(包括延髓、颈髓、上胸部脊髓、胸部脊髓和腰部脊髓)等组织，冷冻保存。 4 、病理观察:采取心脏、肝脏、 ’肾脏、脾脏、肺脏、大脑、脊髓等组织， 4 %甲醛固定，石蜡包埋，切片， he染色，光镜观察。 |
